CPT tube 8ml

A Vacutainer CPT (Cell Preparation Tube) 8mL bụ ọkpọkọ mkpokọta ọbara pụrụ iche nke ejiri maka ikewapụ mkpụrụ ndụ mononuclear (PBMC) na ọbara dum. A na-ejikarị ya na immunology, nchọpụta ọrịa na-efe efe, na ngwa ụlọ ọgwụ dị ka nlekota nke mgbochi na nyocha nke cell.

Akụkụ bụ isi nke Vcutainer CPT Tube (8mL):
Jupụtaburu ụzọ na mgbochi ọbara ọgbụgba na njupụta gradient
Nwere sodium citrate dị ka ihe mgbochi obi.
Gụnyere Ficoll™ nke dabere na njupụta gradient gel nkewa iji kwado nkewa nke sel mononuclear.
Na-akwado ikewapụ otu nzọụkwụ PBMC
Na-ewepụ mkpa maka centrifugation njupụta akwụkwọ ntuziaka ọdịnala (dịka ọmụmaatụ, Ficoll-Paque™).
Na-ebelata oge nhazi na mgbanwe.
Ogo oke olu ọbara
Ezubere iji hazie ihe ruru 8mL nke ọbara dum.
Ihe na-adịghị mma, tube otu eji eme ihe
A na-eji ya maka ngwa ụlọ ọgwụ na nyocha ebe achọrọ ikewapụ PBMC dị elu.
Dakọtara na centrifugation
A chọrọ ụkpụrụ centrifugation kpọmkwem iji kewapụ PBMC nke ọma na akụkụ ọbara ndị ọzọ.

Ojiji na ngwa:
PBMC kewapụrụ maka ọmụmụ ọrụ mgbochi ọrịa (sel T, mkpụrụ ndụ B, mkpụrụ ndụ NK, monocytes).
Cytometry na-efe efe, ELISPOT, nyocha cytokine, na nyocha ọrịa ndị ọzọ.
Ọrịa na-efe efe na nyocha ọgwụ mgbochi.
Nyochaa ọrịa autoimmune.
Nnwale ụlọ ọgwụ na nchọpụta biomarker.

Otu o si arụ ọrụ (Usoro ntọala):
1. Na-anakọta ihe nlele ọbara n'ime tube CPT (jupụta na olu a họpụtara).
2. Tụgharịa nwayọ (ugboro 8-10) iji jikọta ya na ọgwụ mgbochi.
3. Centrifuge dị ka onye na-emepụta ihe si protocol (na-emekarị gburugburu 1500-1800 RCF maka 20-30 nkeji na ụlọ okpomọkụ).
4. PBMC oyi akwa ụdị n'elu gel separator mgbe centrifugation.
5. Jiri nlezianya wepụ oyi akwa PBMC na-eji pipette maka ngwa ala.

Nchekwa na njikwa:
Na-echekwa na ụlọ okpomọkụ (15-25 Celsius C).
Zere oke okpomọkụ ma ọ bụ oyi.